hep 2 slide (Bio-Rad)

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Bio-Rad hep 2 slide

The molecular features of the H‐CDR3 loop are altered in the preB, mB and PCs of mice lacking a pre‐BCR . (A) PCA using H‐CDR3 loop properties for BM B cells from WT (green) and SLC −/− (brown) mice. (B) PC clones, single and HE, were considered alongside BM B cells from SLC −/− mice. (C) Confocal images were obtained by <t>incubating</t> <t>HEp‐2</t> slides with either the 7C9 or the 7C9Mut recombinant antibodies, along with AF488‐coupled goat anti‐IgM and Hoechst. The overview images correspond to maximum‐intensity projections of merged staining. The cells within the marked square were digitally enlarged and analysed using a z‐stack to help discriminate between nuclear and cytoplasmic staining in the xz and yz planes. (D) The H‐CDR3 loops of lengths 3 and 4 aa are depicted in preB and mB cells of SLC −/− mice. Arrows indicate the IRY and LADY H‐CDR3 loops. (E) Model that summarises the properties of the H‐CDR3 loops found in the BM and PC populations of SLC −/− mice. ProB cells: hydrophobic (purple tag) and basic (red tag) loops are prominent. PreB cells: positive charge is more prominent, length is reduced, including a subset with very short loops. mB cells: hydrophobic and positive charge are similar to proB cells, length is similar to preB cells. Single PCs: hydrophobic and positive charge, and length, but not the subset with very short loops areops, are similar to mB. HE‐PC clones: hydrophobic and positive charge, very short length. Positively charged residues are shown in red and hydrophobic residues in purple.

Hep 2 Slide, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hep+2+slides/Kallestad+HEp-2+Slides+(20+x+12+Wells)/pmc13150949-82-1-3
Average 93 stars, based on 5 article reviews

hep 2 slide - by Bioz Stars, 2026-10

93/100 stars

Images

1) Product Images from "Unfavourable H‐CDR3 Loops in preB Cells Lead to Highly Expanded Plasma Cell Clones"

Article Title: Unfavourable H‐CDR3 Loops in preB Cells Lead to Highly Expanded Plasma Cell Clones

Journal: European Journal of Immunology

doi: 10.1002/eji.70200

Figure Legend Snippet: The molecular features of the H‐CDR3 loop are altered in the preB, mB and PCs of mice lacking a pre‐BCR . (A) PCA using H‐CDR3 loop properties for BM B cells from WT (green) and SLC −/− (brown) mice. (B) PC clones, single and HE, were considered alongside BM B cells from SLC −/− mice. (C) Confocal images were obtained by incubating HEp‐2 slides with either the 7C9 or the 7C9Mut recombinant antibodies, along with AF488‐coupled goat anti‐IgM and Hoechst. The overview images correspond to maximum‐intensity projections of merged staining. The cells within the marked square were digitally enlarged and analysed using a z‐stack to help discriminate between nuclear and cytoplasmic staining in the xz and yz planes. (D) The H‐CDR3 loops of lengths 3 and 4 aa are depicted in preB and mB cells of SLC −/− mice. Arrows indicate the IRY and LADY H‐CDR3 loops. (E) Model that summarises the properties of the H‐CDR3 loops found in the BM and PC populations of SLC −/− mice. ProB cells: hydrophobic (purple tag) and basic (red tag) loops are prominent. PreB cells: positive charge is more prominent, length is reduced, including a subset with very short loops. mB cells: hydrophobic and positive charge are similar to proB cells, length is similar to preB cells. Single PCs: hydrophobic and positive charge, and length, but not the subset with very short loops areops, are similar to mB. HE‐PC clones: hydrophobic and positive charge, very short length. Positively charged residues are shown in red and hydrophobic residues in purple.

Techniques Used: Clone Assay, Recombinant, Staining

Staining:

Article Title: Validation of a murine proteome-wide phage display library for identification of autoantibody specificities.
Article Snippet: .. Briefly, Kallestad HEp-2 slides (Bio-Rad) were stained overnight with 1 μL of serum from either Lyn–/– IgD+/– or wild-type mice. .. After washing, antibody binding was detected using an anti-mouse secondary antibody conjugated to Alexa Fluor 488 (Thermo Fisher Scientific) and DAPI counterstain (Thermo Fisher Scientific).

Article Title: Microbial influences on severity and sex bias of systemic autoimmunity.
Article Snippet: .. Presence of anti- nuclear antibodies (ANA) in the serum was tested in samples diluted 1:100 and incubated with HEp- 2 slides (Bio- Rad Laboratories, Hercules, CA) followed by staining with TRITC- α- mouse IgG antibody (Jackson ImmunoResearch). ..

Article Title: Validation of a murine proteome-wide phage display library for identification of autoantibody specificities
Article Snippet: .. Briefly, Kallestad HEp-2 slides (Bio-Rad) were stained overnight with 1 μL of serum from either Lyn –/– IgD +/– or wild-type mice. .. After washing, antibody binding was detected using an anti-mouse secondary antibody conjugated to Alexa Fluor 488 (Thermo Fisher Scientific) and DAPI counterstain (Thermo Fisher Scientific).

Incubation:

Article Title: Androgens contribute to sex bias of autoimmunity in mice by T cell-intrinsic regulation of Ptpn22 phosphatase expression.
Article Snippet: .. HEp-2 slides (Bio-Rad Laboratories, Hercules, CA) were incubated with serum samples diluted at 1:100 in FACS buffer (1% FBS, 0.02% NaN3 in 1XPBS), and counterstained with tetramethylrhodamine-labeled donkey anti-mouse IgG (Jackson ImmunoResearch, West Grove, PA, cat#715-025-151, Lot# 59965)) diluted at 1:100 in FACS buffer. ..

Article Title: Androgens contribute to sex bias of autoimmunity in mice by T cell-intrinsic regulation of Ptpn22 phosphatase expression
Article Snippet: .. HEp-2 slides (Bio-Rad Laboratories, Hercules, CA) were incubated with serum samples diluted at 1:100 in FACS buffer (1% FBS, 0.02% NaN3 in 1XPBS), and counterstained with tetramethylrhodamine-labeled donkey anti-mouse IgG (Jackson ImmunoResearch, West Grove, PA, cat#715-025-151, Lot# 59965)) diluted at 1:100 in FACS buffer. ..

FACS:

Immunofluorescence:

Article Title: Activation of autoreactive lymphocytes in the lung by radioresistant cells expressing a STING gain-of-function mutation
Article Snippet: Microscopy was captured on a Leica SP8 confocal microscope or a Leica Thunder widefield fluorescence microscope and analyzed in the Leica Application Suite X. .. Sera diluted 1:25 in 0.2% BSA PBS were evaluated by immunofluorescence microscopy using HEp-2 slides (Bio-Rad, 26102). mAbs PL2-3 (IgG2a) and 7D7 (IgM) were used as positive controls (2 mg/mL). .. Bound antibodies were detected with goat anti–mouse IgG-Dylight 488 (Invitrogen, A-11001) or goat anti–mouse IgM-Dylight 488 (Invitrogen, SA5-10150).

Microscopy:

Article Title: Activation of autoreactive lymphocytes in the lung by STING gain-of-function mutation expressing radioresistant cells.
Article Snippet: Microscopy was captured on a Leica SP8 confocal microscope or a Leica Thunder widefield fluorescent microscope and analyzed in the Leica Application Suite X. Anti-nuclear antibody assay. .. Sera diluted 1:25 in 0.2% BSA PBS were evaluated by immunofluoescent microscopy using HEp-2 slides (Biorad #26102). mAbs PL2-3 (IgG2a) and 7D7 (IgM) were used as positive controls (2mg/ml). .. Bound antibodies were detected with goat anti-mouse IgG-Dylight 488 (Invitrogen #A11001) or goat anti-mouse IgM-Dylight 488 (Invitrogen #SA5-10150).

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